pet-14b vector (Millipore)
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pet-14b vector
Pet 14b Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-14b+vector/pet28a/pm38874471-33-20-22
Average 90 stars, based on 1 article reviews
Pet 14b Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-14b+vector/pet28a/pm38874471-33-20-22
Average 90 stars, based on 1 article reviews
pet-14b vector - by Bioz Stars,
2026-09
90/100 stars
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Construct:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Modification:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Plasmid Preparation:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Sequencing:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Clone Assay:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Polymerase Chain Reaction:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. cDNA Library Assay:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Purification:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Gel Extraction:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Amplification:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Expressing:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. SDS Page:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Transfection:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Electrophoresis:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. Agarose Gel Electrophoresis:Article Title: Cooperative Gsx2-DNA Binding Requires DNA Bending and a Novel Gsx2 Homeodomain Interface Article Snippet: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Outer membrane protein OmpV mediates Salmonella enterica serovar typhimurium adhesion to intestinal epithelial cells via fibronectin and α1β1 integrin. Article Snippet: Funding information Indian Institute of Science Education and Research Mohali Abstract Salmonella typhimurium is an invasive Gram-negative enteric bacterium, which causes salmonellosis, a type of gastroenteritis in humans and typhoid-like symptoms in mice.. Upon entering through the contaminated food and water, S. typhimurium adheres, colonises, and invades intestinal epithelial cells (IECs) of the small intestine.. In this study, we have shown that upon deletion of the outer membrane protein OmpV, there is a significant decrease in adherence of S. typhimurium to the IECs, indicating that OmpV is an important adhesin of S. typhimurium. Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease Article Snippet: The PCR was performed for 30 cycles of 95 °C for 30 s, 66 °C for 30 s and 72 °C for 60 s. The PCR product was gel purified by Qiaquick gel extraction kit (Qiagen), digested with Nde I and BamH I (New England Biolabs) and ligated into a Article Title: Insight into the C-terminal SH3 domain mediated binding of Drosophila Drk to Sos and Dos. Article Snippet: Cloning, expression, and purification of Drk-CSH3 The gene encoding Drk-CSH3 (residues, 153e211) was cloned into Article Title: A Type IIa crustin from the pink shrimp Farfantepenaeus paulensis (crusFpau) is constitutively synthesized and stored by specific granule-containing hemocyte subpopulations. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Article Title: Cooperative Gsx2-DNA binding requires DNA bending and a novel Gsx2 homeodomain interface. Article Snippet: All Gsx2 constructs were subcloned from a complementary DNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector ( Article Title: Thermodynamics of amyloid fibril formation from non-equilibrium experiments of growth and dissociation. Article Snippet: Amyloid fibrils are ordered, non-covalent polymers of proteins that are linked to a range of diseases, as well as biological functions.. Amyloid fibrils are often considered thermodynamically so stable that they appear to be irreversible, explaining why very few quantitative thermodynamic studies have been performed on amyloid fibrils, compared to the very large body of kinetic studies.. Here we explore the thermodynamics of amyloid fibril formation by the protein PI3K-SH3, which forms amyloid fibrils under acidic conditions. Article Title: NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes Article Snippet: Genes encoding yeast S. cerevisiae Oxa1 (NCBI reference sequence: NM_001179044) were cloned by Polymerase Chain Reaction into the pET-14b vector (Novagen, Darmstadt, Germany) with a N-terminal 6× His-tag and a thrombin cleavage site, using the yeast Saccharomyces cerevisiae cDNA library as a template. |